Fictional characters

• Twilight (Marvel Comics), in the Marvel Comics X-Nation 2099 series
• Twilight, in the DP 7 comic book series
• Twilight, a persona of Angel (Buffy the Vampire Slayer)
• Twilight, in the Milestone Comics superhero team Shadow Cabinet
• Twilight, a great grey owl in the Guardians of Ga'Hoole novel series by Kathryn Lasky
• Twilight, from Go! Princess Precure
• Twilight Sparkle, the main character of My Little Pony: Friendship Is Magic
• Sophie Twilight, from Ms. Vampire Who Lives in My Neighborhood
• Twilight, the main character of the manga series Spy × Family

Film

• Twilight (1940 film), a German film by Rudolf van der Noss
• Twilight (1944 film), a French drama film directed by Marc Allégret
• Twilight (1945 film), a Mexican drama film directed by Julio Bracho
• Twilight (1969 film), a Cambodian film by King Norodom Sihanouk
• Twilight (1998 film), an American thriller by Robert Benton
• Twilight (2007 film), a.k.a. La Brunante, a Canadian drama film directed by Fernand Dansereau
• The Twilight Story , a 1960 Japanese film by Shirō Toyoda
• The Twilight Saga (film series), adapted from Stephenie Meyer's novels
  • Twilight (2008 film), the first film in the series

Television

• "Twilight" (NCIS), 2005
• "Twilight" (Star Trek: Enterprise), 2003
• "Twilight", a 1989 episode of China Beach
• "Twilight", a 2008 episode of Di-Gata Defenders
• "Twilight", a 2003 episode of The Galaxy Railways
• "Twilight", a 1998 episode of Hercules: The Legendary Journeys
• "Twilight", a 1998 episode of Lexx
• "Twilight", a 2006 episode of Rescue Me
• "Twilight", a 2003 episode of Six Feet Under
• "Twilight", a 2003 episode of Justice League

Books

• Twilight (novel series), a series of novels by Stephenie Meyer
  • Twilight (Meyer novel), the first novel in the series
• Twilight, a novella by Stefan Zweig, 1910
• Twilight, a novel by Frank Danby, 1916
• "Twilight" (Campbell short story), a short story by John W. Campbell, 1934
• Twilight, a novel by Yosef Reinman, published under the pen name Avner Gold, 1985
• Twilight, a novel by Judith Arnold, 1988
• Twilight (Wiesel novel), a novel by Elie Wiesel, 1988
• Twilight: Where Darkness Begins , a 1980s series of teen horror novels
• Twilight, a novel by Peter James, 1991
• Twilight, a novel by Nancy Pickard, 1995
• Twilight, a novel by Sherryl Woods, 1997
• Twilight (Cabot novel), the sixth novel in the Mediator series by Meg Cabot, 2004
• Twilight, a novel by William Gay, 2006
• Twilight, a novel by Brendan DuBois, 2007
• Twilight (Hunter novel), a 2007 Warriors: The New Prophecy novel by Erin Hunter
• Twilight, a novel by Azhar Abidi, 2008
• Doors X: Twilight, a novel by Markus Heitz, 2018
• The Servants of Twilight , a 1984 novel by Dean Koontz, originally released as Twilight

Comics

• Twilight (comic book), a 1990 DC Comics miniseries by Howard Chaykin and Jose Luis Garcia Lopez
• "Twilight" (Buffy comic), a 2010 story arc of the Buffy the Vampire Slayer Season Eight series
• Twilight: The Graphic Novel , a 2010 2-part comic book miniseries by Young Kim
• Twilight, a "bad girl" comic from Avatar Press
• Twilight, a story arc of the Star Wars: Republic series

Music

Visual arts

• Twilight (painting), a 1981 painting by Odd Nerdrum
• Twilight, a series of photographs by Gregory Crewdson

Other uses in arts and entertainment

• Twilight (game developer), a defunct UK video game development group
• Twilight: 2000 , a 1984 role-playing game
• Twilight: Los Angeles, 1992 , a 1994 play by Anna Deavere Smith
• Project: Twilight, a 2001 audio drama based on the TV series Doctor Who