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Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)

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Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)
Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II).png
Identifiers
3D model (JSmol)
ECHA InfoCard 100.120.143 OOjs UI icon edit-ltr-progressive.svg
EC Number
  • 608-445-8
  • InChI=1S/3C24H16N2O6S2.4Na.Ru/c3*27-33(28,29)17-5-1-15(2-6-17)19-11-13-25-23-21(19)9-10-22-20(12-14-26-24(22)23)16-3-7-18(8-4-16)34(30,31)32;;;;;/h3*1-14H,(H,27,28,29)(H,30,31,32);;;;;/q;;;4*+1;+2/p-6
    Key: AXMFYAOBYBXMLC-UHFFFAOYSA-H
  • C1=CC(=CC=C1C2=C3C=CC4=C(C=CN=C4C3=NC=C2)C5=CC=C(C=C5)S(=O)(=O)[O-])S(=O)(=O)[O-].C1=CC(=CC=C1C2=C3C=CC4=C(C=CN=C4C3=NC=C2)C5=CC=C(C=C5)S(=O)(=O)[O-])S(=O)(=O)[O-].C1=CC(=CC=C1C2=C3C=CC4=C(C=CN=C4C3=NC=C2)C5=CC=C(C=C5)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Ru+2]
Properties
C72H42N6Na4O18RuS6
Molar mass 1664.54 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II) (Na4Ru(bps)3) is a sodium salt of coordination complex. In this form, it is the salt of a sulfonic acid. This compound or related salts are classified as transition metal complexes of 1,10-phenanthroline. Ruthenium(II) tris(bathophenanthroline disulfonate), referring to the anionic fragment, is used as a protein dye in biochemistry to differentiate and detect proteins. In 2-D electrophoresis the complex is used in a standard procedure to separate complex protein mixtures in proteome studies (Proteomics). [1] [2] and a step in gene expression profiling.

Contents

For protein detection, it is advantageous to use fluorescent labels containing chromophores that have longer excitation wavelength and emission wavelength than the aromatic amino acids. These dyes combine good signal to background ratio (contrast), broad linear dynamic range, broad application range, photochemical stability, and compatibility to protein identification techniques, e.g. mass spectrometry (MS) or Western blotting.

History

Tris(bathophenanthroline disulfonate)ruthenium(II) was synthesized by Bannwarth [3] as a dye for oligo nucleotides. [4] Within 5 years, similar transition metal complexes had been recognized as workable protein detection reagents, [5] and shortly afterwards the europium analog of RuBPS was demonstrated as an effective fluorescent protein detection reagent. [6] The first reported use of RuBPS for protein detection appears to be the commercial release of the proprietary Sypro Ruby protein staining solution in 1999. [7] [8] While Sypro Ruby is proprietary & is not stated to have RuBPS as the major component, it is stated to have ruthenium, and Rabilloud et al. synthesized RuPBS and compared it to Sypro Ruby, finding them to be highly similar, albeit not identical, reagents for fluorescent detection of proteins in polyacrylamide gels. [7] [9] Notably, Rabilloud et al. made their comparisons against the first formulation of Sypro Ruby, the second (and presumed current) formulation of Sypro Ruby has the same product numbers (but distinct lot numbers) and an increased performance with diverse fixative solutions. [10] [11] The fact that RuBPS is not only easy to synthesize but also easy to handle, induced further developments in this field.

RuBPS staining protocol by selectively destaining the polyacrylamide matrix while the protein content remained tinctured. This technique offers a variety of advantages. [11] Related ruthenium(II) complexes have some improved properties, specifically a broader pH range where they could be used. [12]

References

  1. ↑ Miller, I.; Crawford, J.; Gianazza, E. (October 2006). "Protein stains for proteomic applications: Which, when, why?". Proteomics . 6 (20): 5385–5408. doi: 10.1002/pmic.200600323 . PMID   16991193. S2CID   27311378.
  2. ↑ Lamanda, Andreas; Cheaib, Zeinab; Turgut, Melek Dilek; Lussi, Adrian (2007). Imhof, Axel (ed.). "Protein Buffering in Model Systems and in Whole Human Saliva". PLoS ONE . 2 (2): e263. Bibcode:2007PLoSO...2..263L. doi: 10.1371/journal.pone.0000263 . PMC   1803027 . PMID   17327922. Open Access logo PLoS transparent.svg
  3. ↑ "Archived copy". Archived from the original on 2007-05-15. Retrieved 2007-04-26.{{cite web}}: CS1 maint: archived copy as title (link)
  4. ↑ Bannwarth, W.; Schmidt, D.; Stallard, R. L.; Hornung, C.; Knorr, R.; Müller, F. (December 1988). "Bathophenanthroline-ruthenium(II) complexes as non-radioactive labels for oligonucleotides which can be measured by time-resolved fluorescence techniques". Helvetica Chimica Acta . 71 (8): 2085. doi:10.1002/hlca.19880710826.
  5. ↑ Patton, W.F.; Lam, L.; Su, Q.; Lui, M.; Erdjumentbromage, H.; Tempst, P. (1994). "Metal Chelates as Reversible Stains for Detection of Electroblotted Proteins: Application to Protein Microsequencing and Immunoblotting". Analytical Biochemistry. 220 (2). Elsevier BV: 324–335. doi: 10.1006/abio.1994.1345 . ISSN   0003-2697. PMID   7526738. S2CID   24405621.
  6. ↑ Lim, Mark J.; Patton, Wayne F.; Lopez, Mary F.; Spofford, Kimberley H.; Shojaee, Negin; Shepro, David (1997). "A Luminescent Europium Complex for the Sensitive Detection of Proteins and Nucleic Acids Immobilized on Membrane Supports". Analytical Biochemistry. 245 (2). Elsevier BV: 184–195. doi: 10.1006/abio.1996.9961 . ISSN   0003-2697. PMID   9056210.
  7. 1 2 Berggren, Kiera; Steinberg, Thomas H.; Lauber, Wendy M.; Carroll, James A.; Lopez, Mary F.; Chernokalskaya, Elena; Zieske, Lynn; Diwu, Zhenjun; Haugland, Richard P.; Patton, Wayne F. (1999). "A Luminescent Ruthenium Complex for Ultrasensitive Detection of Proteins Immobilized on Membrane Supports". Analytical Biochemistry. 276 (2). Elsevier BV: 129–143. doi:10.1006/abio.1999.4364. ISSN   0003-2697. PMID   10603235.
  8. ↑ Steinberg, Thomas H.; Chernokalskaya, Elena; Berggren, Kiera; Lopez, Mary F.; Diwu, Zhenjun; Haugland, Richard P.; Patton, Wayne F. (2000-02-01). "Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain". Electrophoresis. 21 (3). Wiley: 486–496. doi:10.1002/(sici)1522-2683(20000201)21:3<486::aid-elps486>3.0.co;2-q. ISSN   0173-0835. PMID   10726748. S2CID   36787237.
  9. ↑ "Proteome - Full Text DOI 10.1007/S102160000002". doi:10.1007/S102160000002. S2CID   98713308. Archived from the original on 2011-05-29. Retrieved 2007-04-26.{{cite journal}}: Cite journal requires |journal= (help)
  10. ↑ Berggren, Kiera N.; Schulenberg, Birte; Lopez, Mary F.; Steinberg, Thomas H.; Bogdanova, Alla; Smejkal, Gary; Wang, Annie; Patton, Wayne F. (2002). "An improved formulation of SYPRO Ruby protein gel stain: Comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation". Proteomics. 2 (5). Wiley: 486–498. doi:10.1002/1615-9861(200205)2:5<486::aid-prot486>3.0.co;2-x. ISSN   1615-9853. PMID   11987123. S2CID   23902055.
  11. 1 2 Lamanda, A.; Zahn, A.; Röder, D.; Langen, H. (March 2004). "Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal-to-background ratio and improved baseline resolution". Proteomics . 4 (3): 599–608. doi:10.1002/pmic.200300587. PMID   14997483. S2CID   26442102.
  12. ↑ Babak, Maria V.; Le Faouder, Pauline; Trivelli, Xavier; Venkatesan, Gopalakrishnan; Bezzubov, Stanislav I.; Kajjout, Mohammed; Gushchin, Artem L.; Hanif, Muhammad; Poizat, Olivier; Vezin, Hervé; Rolando, Christian (2020-03-17). "Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining". Inorganic Chemistry. 59 (7). American Chemical Society (ACS): 4527–4535. doi:10.1021/acs.inorgchem.9b03679. ISSN   0020-1669. PMID   32181663. S2CID   212739460.

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