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Stephen Bustin

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Stephen Andrew Bustin (born 1954) [1] is a British scientist, former professor of molecular sciences at Queen Mary University of London from 2004 to 2012, as well as visiting professor at Middlesex University, beginning in 2006. [2] [3] In 2012 he was appointed Professor of Allied Health and Medicine at Anglia Ruskin University. [4] [ failed verification ] He is known[ by whom? ] for his research into polymerase chain reaction.

Contents

Education

Bustin obtained his B.A. and PhD from Trinity College, Dublin in molecular genetics. [5] [ dead link ]

Career

In 2023, Bustin was elected a member of the Academia Europaea. [6] [ non-primary source needed ]

Research

His research group’s general areas of interest are the small and large bowel, as well as colorectal cancer with particular emphasis on investigating the process of invasion and metastasis. An important aim is to translate molecular techniques into clinical practice by including molecular parameters into clinical tumor staging. [7] To this end, Bustin has published many papers on PCR techniques, [8] [9] [ failed verification ] in particular reverse transcription polymerase chain reaction, the subject of his most cited paper, published in 2000. [10]

Bustin led a group who the MIQE guidelines in a 2009 paper published in Clinical Chemistry , [11] the goal of which is to create guidelines for how PCR should be performed to ensure that PCR results are being reliably conducted and interpreted, as well as to make replication of experiments easier. This paper is the fifth most cited one ever to be published in Clinical Chemistry, [12] [13] with over 17000 citations on Google Scholar as of September 2025. [14]

Lundy murders testimony

Bustin testified in the trials pertaining to the Lundy murders in 2015, criticizing tests that had claimed to detect human brain cells on Mark Lundy's shirt. [15] [16]

References

  1. ↑ "Stephen Bustin". ResearchGate . Retrieved 24 September 2013.
  2. ↑ "Stephen Bustin's Biography". Selectbiosciences. 2011. Retrieved 12 August 2013.
  3. ↑ McGee, Patrick (10 May 2007). "How Reliable is Your qPCR Data?". Drug Discovery & Development. Retrieved 12 December 2013.
  4. ↑ Bustin, Stephen (2013). "Definitive qPCR" . Retrieved 12 August 2013.
  5. ↑ "Stephen Bustin". Anglia Ruskin University. Retrieved 30 September 2016.
  6. ↑ "Stephen Bustin". Member. Academia Europaea. Retrieved 2 November 2024.
  7. ↑ "The future of qPCR". American Association for the Advancement of Science. 30 September 2010. Retrieved 11 August 2013.
  8. ↑ Nolan, T.; Hands, R. E.; Bustin, S. A. (2006). "Quantification of mRNA using real-time RT-PCR". Nature Protocols. 1 (3): 1559–1582. doi:10.1038/nprot.2006.236. PMID   17406449. S2CID   10108148.
  9. ↑ Mueller, R.; Bustin, S. A. (2005). "Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis". Clinical Science. 109 (4): 365–379. CiteSeerX   10.1.1.328.8607 . doi:10.1042/CS20050086. PMID   16171460.
  10. ↑ Bustin, S. (2000). "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays". Journal of Molecular Endocrinology. 25 (2): 169–193. doi: 10.1677/jme.0.0250169 . PMID   11013345.
  11. ↑ Bustin, S. A.; Benes, V.; Garson, J. A.; Hellemans, J.; Huggett, J.; Kubista, M.; Mueller, R.; Nolan, T.; Pfaffl, M. W.; Shipley, G. L.; Vandesompele, J.; Wittwer, C. T. (2009). "The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments". Clinical Chemistry. 55 (4): 611–622. doi: 10.1373/clinchem.2008.112797 . PMID   19246619.
  12. ↑ "The MIQE Guidelines" . Retrieved 11 August 2013.
  13. ↑ "Most Cited Articles". Clinical Chemistry . Retrieved 9 September 2013.
  14. ↑ "The MIQE Guidelines: M inimum I nformation for Publication of Q uantitative Real-Time PCR e xperiments".
  15. ↑ Quilliam, Rebecca (11 March 2015). "Mark Lundy retrial: DNA on shirt matched to victim". New Zealand Herald . Retrieved 30 August 2015.
  16. ↑ Galuszka, Jono (9 March 2015). "Mark Lundy murder retrial: Day 20". Stuff.co.nz . Retrieved 30 August 2015.
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