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Demethylrebeccamycin-D-glucose O-methyltransferase

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Demethylrebeccamycin-D-glucose O-methyltransferase
Identifiers
EC no. 2.1.1.164
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
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PMC articles
PubMed articles
NCBI proteins

Demethylrebeccamycin-D-glucose O-methyltransferase (EC 2.1.1.164, RebM) is an enzyme with systematic name S-adenosyl-L-methionine:demethylrebeccamycin-D-glucose O-methyltransferase. [1] [2] This enzyme catalyses the following chemical reaction

+ SAM
 
 
 
 
Demethylrebeccamycin-D-glucose O-methyltransferase
 
 
 
+ SAH
 

This is the final step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid isolated from Nocardia bacteria. [3] [4] It uses S-adenosyl methionine as a cofactor. [5]

References

  1. Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS (May 2006). "RebG- and RebM-catalyzed indolocarbazole diversification". ChemBioChem. 7 (5): 795–804. doi:10.1002/cbic.200500504. PMID   16575939. S2CID   25017820.
  2. Singh S, McCoy JG, Zhang C, Bingman CA, Phillips GN, Thorson JS (August 2008). "Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebM". The Journal of Biological Chemistry. 283 (33): 22628–36. doi: 10.1074/jbc.M800503200 . PMC   2504894 . PMID   18502766.
  3. Bush JA, Long BH, Catino JJ, Bradner WT, Tomita K (May 1987). "Production and biological activity of rebeccamycin, a novel antitumor agent". The Journal of Antibiotics. 40 (5): 668–78. doi: 10.7164/antibiotics.40.668 . PMID   3112080.
  4. Pommerehne, Kathrin; Walisko, Jana; Ebersbach, Anna; Krull, Rainer (2019). "The antitumor antibiotic rebeccamycin—challenges and advanced approaches in production processes". Applied Microbiology and Biotechnology. 103 (9): 3627–3636. doi:10.1007/s00253-019-09741-y. PMID   30888461.
  5. Enzyme 2.1.1.164 at KEGG Pathway Database.
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