Fictional characters

• Twins (The Matrix)
• The Twins (Transformers)
• Leonel and Marco Salamanca, Breaking Bad and Better Call Saul characters referred to as "The Twins"
• The Twins, or Spazmatism and Retinazer, a pair of bosses in the video game Terraria

Films

• The Twins (1923 film), an Australian silent film
• Twins (1925 film), an American comedy film starring Stan Laurel
• Twins (1988 film), an American comedy film starring Arnold Schwarzenegger and Danny DeVito
• The Twins (2005 film), a South Korean film
• The Twin (2022 film), a psychological horror film

Literature

• Twins (novel), a 2006 young adult novel by Marcy Dermansky
• Twins, a 1977 novel by Jack Geasland and Bari Wood, based on the story of Stewart and Cyril Marcus
• Twins, a series of 26 children's books by Lucy Fitch Perkins
• Twins, a 1999 photobook by Steven Underhill
• The Twins (De Loo novel), by Tessa de Loo, 1993
• The Twins (1930 novel), a novel by Abd Al Quddus Al Ansari
• "The Twins", a poem by Henry Sambrooke Leigh (1837–1883)
• Twins: A Variety Store Named "The End of the World", a novel by Novala Takemoto
• "The Twins" (folktale), an Albanian folktale

Music

Television

• Twins (TV series), a 2005 American sitcom
• The Twins (1979 TV series), a Hong Kong television series
• "Twins" (Doctors), a 2003 episode
• "The Twins" (Star Wars: Visions), a 2021 episode