The Prime Directive is a guiding principle of Starfleet, an organization in the fictional universe of the Star Trek media franchise.

Prime Directive may also refer to:

• Prime Directive (role-playing game), by the Amarillo Design Bureau
• Prime Directive (album), a 1999 album by the Dave Holland Quintet
• Prime Directive (novel), a 1990 Star Trek novel by Judith and Garfield Reeves-Stevens
• Prime directive (RoboCop), four rules governing the behavior of RoboCop in the fictional media franchise
• A fake working title of the Transformers (film series)
• Prime Directive, a concept in the 1947 science fiction novelette "With Folded Hands ..." by Jack Williamson

This disambiguation page lists articles associated with the title Prime Directive.
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