In public transport, Route 1 may refer to:
- Route 1 (MTA Maryland), a bus route in Baltimore, Maryland
- Route 1 (Malta), a bus route in Malta
- Barcelona Metro line 1
- Line 1 (Beijing Subway)
- Line 1 (Hangzhou Metro)
- Citybus Route 1 in Hong Kong
- KMB Route 1 in Hong Kong
- London Buses route 1
- Manila LRT Yellow Line
- Line 1 Green (Montreal Metro)
- 1 (New York City Subway service)
- Seoul Subway Line 1
- 1 California (bus line) in San Francisco
- Shanghai Metro Line 1
- Line 1 (Shenzhen Metro)
- Melbourne tram route 1
- Line 1 Yonge-University, in Toronto
- Transjakarta Corridor 1 Blok M-Kota
This set index article includes a list of related items that share the same name (or similar names).