Awards

• ACE Awards (Award for Cable Excellence)

Comics

• Ace Comics , a 1937-1959 comic book series
• Ace Magazines (comics), a 1940-1956 a comic-book and pulp-magazine publishing company

Music

Video games

• A.C.E. Another Century's Episode , a 2005 video game for the PlayStation 2

Other uses in arts, entertainment, and media

• Ace Cinema, cinema in the London Borough of Harrow
• Ace (film)
• ACE (games magazine)
• Ace (musical) 2006
• Ace (Wild Cards), a class of characters in the Wild Cards science fiction universe

Aerospace

• Advanced Composition Explorer, a NASA satellite
• Advanced Crew Escape Suit, an astronaut suit
• Advanced Cryogenic Evolved Stage, a proposed rocket 2nd stage for ULA
• Asia Cellular Satellite system, a precursor of the Indonesian satellite company PT Pasifik Satelit Nusantara
• Atmospheric Chemistry Experiment, a project of the Canadian satellite SCISAT-1
• Atomic Clock Ensemble in Space, a European Space Agency project

Biology, chemistry, and healthcare

• ACE inhibitor, a drug that lowers blood pressure
• Ace K, acesulfame potassium, an artificial sweetener
• A.C.E. mixture, an historical anesthetic
• Acepromazine, an antipsychotic drug used primarily in animals
• ACES (buffer), one of Good's buffers
• ACES (nutritional supplement), containing vitamins A, C, E and selenium
• ACE unit (Acute Care of Elderly), a type of hospital facility
• Addenbrooke's Cognitive Examination
• Adverse childhood experiences
• Angiotensin-converting enzyme, a central component of the renin–angiotensin system (RAS), which controls blood pressure

Computing

• Access Control Entry, part of an access control list
• ACE (compressed file format)
• Ace (editor), a code editor written in JavaScript
• ACE (editor), a desktop-based collaborative editor
• ACE (genomic file format)
• Acehnese language (ISO 639 code)
• ACES (computational chemistry), a software package
• Adaptive Communication Environment, an open-source network-programming framework
• Advanced Computerized Execution System, a stock-trading system
• Advanced Computing Environment, an early 1990s initiative for commodity computing hardware
• Agent-based computational economics
• Arbitrary code execution
• ASCII Compatible Encoding, a prefix related to internationalized domain names
• Asynchronous Compute Engine, a component defined in AMD's Graphics Core Next for ATI-type products
• Automatic Computing Engine, a 1952 British computer
• Automatic Content Extraction, an information-extraction technology-development program
• Jupiter Ace, a 1980s British computer
• Anti-Cheat Expert, an anti-cheat system developed by Tencent

Other uses in science and technology

• Academy Color Encoding System (ACES), a proposed color image encoding
• Accumulated cyclone energy, commonly known as (ACE), metric used by various agencies to express the energy released by a tropical cyclone
• ACE (cable system), a submarine communications cable
• ACE experiment, the Antiproton Cell Experiment hosted at CERN
• Ace (quark model), an obsolete term for quark
• Aggregative Contingent Estimation, a program of the Intelligence Advanced Research Projects Agency
• Alternating conditional expectations, an algorithm in nonparametric regression.
• Alternative Chassis Engineering, a UK bus manufacturer, builder of the ACE Cougar
• Attempto Controlled English, a controlled natural language from the University of Zurich

Terminology

• Ace (baseball), a team's best starting pitcher
• Ace formation, in American football
• Ace (golf), a hole in one
• Ace (pickleball), a serve that is not returned by the receiver
• Ace (tennis), a point won by the server without contest

Teams

• Alaska Aces (ECHL), an American ice hockey team
• Alaska Aces (PBA), a Philippine basketball team
• Alexandria Aces, a defunct American baseball team in Louisiana
• Asheville Aces, a defunct American ice hockey team
• Belle Vue Aces, an English motorcycle speedway team
• Cornwall Aces, a Canadian ice hockey team playing 1993-1996
• Dallas Aces, the world's first professional bridge team
• Evansville Purple Aces, a college team of the University of Evansville
• Las Vegas Aces, an American basketball team
• Lower Merion High School Aces, the athletic teams at the school in suburban Philadelphia
• Melbourne Aces, an Australian baseball team founded 2009
• Northeast League Aces, a Canadian-American baseball team
• Quebec Aces, a Canadian ice hockey team playing 1928-1971
• Reno Aces, an American baseball team in Nevada
• Victoria Aces, an Australian baseball team founded 1934

Automotive

• AC Ace, a 1953-1963 English car
• Ace Motor Corporation, a 1919–1927 American motorcycle company
• Ace (1913 automobile), a 1912-1916 British car
• Ace (1920 automobile), a 1920-1922 American car
• Atlantic City Expressway, a New Jersey toll road which is the main route between Atlantic City and Philadelphia
• Tata Ace, an Indian mini-truck introduced 2005

Rail

• Altamont Corridor Express, a California train service
• Atlantic City Express Service, a New York-New Jersey train service
• Atlantic Coast Express, an England train service
• EMD SD70ACe, an American diesel-electric locomotive commonly referred to as an "Ace"
• Kintetsu 22600 series "Ace", a Japanese train type
• IND Eighth Avenue Line, New York City Subway line served by the A, C, and E trains