In enzymology, an ADP-L-glycero-D-manno-heptose 6-epimerase (EC 5.1.3.20) is an enzyme that catalyzes the chemical reaction

ADP-D-glycero-D-manno-heptose ${\displaystyle \rightleftharpoons }$ ADP-L-glycero-D-manno-heptose

Hence, this enzyme has one substrate, ADP-D-glycero-D-manno-heptose, and one product, ADP-L-glycero-D-manno-heptose.

This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is ADP-L-glycero-D-manno-heptose 6-epimerase. This enzyme participates in lipopolysaccharide biosynthesis. It employs one cofactor, NADP+ in a direct oxidation mechanism.

Structural studies

As of 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1EQ2.

References

• Read JA, Ahmed RA, Morrison JP, Coleman WG Jr, Tanner ME (2004). "The Mechanism of the Reaction Catalyzed by ADP-β-L-glycero-D-manno-heptose 6-Epimerase". J. Am. Chem. Soc. 126 (29): 8878–9. Bibcode:2004JAChS.126.8878R. doi:10.1021/ja0485659. PMID   15264802.
• Ding L, Seto BL, Ahmed SA, Coleman WG Jr (1994). "Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosugar epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase". J. Biol. Chem. 269 (39): 24384–90. doi: 10.1016/S0021-9258(19)51095-X . PMID   7929099.
• Raetz CR (1990). "Biochemistry of endotoxins". Annu. Rev. Biochem. 59: 129–70. doi:10.1146/annurev.bi.59.070190.001021. PMID   1695830.